Figure 4 | Scientific Reports

Figure 4

From: Genetic disruption of Plasmodium falciparum Merozoite surface antigen 180 (PfMSA180) suggests an essential role during parasite egress from erythrocytes

Figure 4

PfMSA180-derived recombinant proteins do not bind erythrocytes and MSA180-specific antibodies do not inhibit invasion of erythrocytes by P. falciparum in vitro. (a) Recombinant PfMSA180 proteins fail to bind erythrocytes in a FACS-based binding assay. 10 µg of each recombinant protein was used. EBA175 region II and CyRPA were used as positive and negative controls. One of two experiments is shown. (b) Antibodies raise against PfMSA180 recombinant proteins failed to inhibit erythrocyte invasion in an in vitro Growth Inhibition Assay (GIA). 10 mg/ml of purified rabbit antibody were used and each exhibited < 5% inhibition. Rabbit antibodies targeting CyRPA were used as a positive control. Three independent assays were performed in duplicate. The error bars show the standard error of the mean.

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