Figure 5
Histograms representing the effect of 3 h in vitro incubation (37 °C, 5%CO2) of whole semen samples on sperm mitochondrial ROS production, measured by Mitosox Red, with all tested bacterial strains (P. aeruginosa ATCC 27853, E. coli ATCC 25922, E. coli ATCC 35218, K. quasipneumoniae ATCC 700603, K. pneumoniae ATCC 13883, E. cloacae ATCC 13047 and K. aerogenes ATCC 13048) (n = 6, panel A), sperm caspases 3 and 7 activity, measured by FLICA, with E. coli ATCC 25922, E. coli ATCC 35218 and K. aerogenes ATCC 13048 (n = 5, panel B) and sperm DNA fragmentation, measured by TUNEL/PI, with E. coli ATCC 25922, E. coli ATCC 35218, K. quasipneumoniae ATCC 700603 and K. aerogenes ATCC 13048 (n = 5, panel C). Student t-test, *p < 0.05; #p < 0.01.
