Figure 2 | Scientific Reports

Figure 2

From: Differential regulation of mammalian and avian ATOH1 by E2F1 and its implication for hair cell regeneration in the inner ear

Figure 2

Effect of the E2F transcription factors on the mouse and chick Atoh1 enhancers and putative enhancer C. (A) Schematic representation of the luciferase constructs containing the mouse or chick Atoh1 conserved regions cloned into the luciferase vector pGL4.23. (B) Dose–response effects on the Atoh1 luciferase constructs of transfecting increasing amounts of an E2F1 expression construct or mutant E2F1 in the UB/OC-2 cell line. (C) Effect of E2F2, E2F3 and E2F4 on the activity of the Atoh1 luciferase constructs. Experiments were conducted in triplicate in two separate assays with different DNA preparations for each plasmid in each assay (n = 6). Relative luciferase values are expressed relative to activity of the reporters in the absence of E2F. Student t-test was conducted comparing each condition with 0 ng of the corresponding E2F expression construct. (*p < 0.05; **p < 0.01; #p < 10–5; ##p < 10–10). Error bars represent the s.e.m (n = 6).

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