Table 3 List of candidate reference genes and the corresponding primer parameters used in this study.
Gene symbol | Primer sequences (5'–3') | PCR product length (bp) | Tm of PCR products (°C) | Primer design | PCR amplification efficiency (%) | Regression Coefficient (R-squared) |
|---|---|---|---|---|---|---|
SNW1 | Fw: GCAGCTCCTGATAAGAGGTCG Rev: CCGAGGATTAGGAACACCGAG | 87 | 78.0 | Intron-spanning | 95.8 | 0.9996 |
CNOT4 | Fw: GTCCAAAACCTGACTGCATGTATC Rev: GGTGTTTACCCGCCTGCAT | 87 | 80.8 | Intron-spanning | 96.3 | 0.9999 |
PUM1 | Fw: TGCGGGAGATTGCTGGACAT Rev: GTGTGGCACGCTCCAGTTTC | 87 | 80.4 | Intron-flanking | 98.4 | 0.9999 |
PCBP1 | Fw: ATTCGCCGGAATTGACTCCA Rev: TGCCCAATAGCCTTTCACCT | 49 | 86.4 | Exonic | 99.8 | 0.9998 |
IPO8 | Fw: GGCATACAGTTTAACCTGCCAC Rev: CAGGAGAGGCATCATGTCTGTAA | 118 | 78.6 | Intron-spanning | 92.5 | 0.9995 |
HNRNPL | Fw: CCAAGGCCTCTCTCAATGGG Rev: TTCAAGCGTGTAGGCTTTGC | 82 | 80.0 | Intron-spanning | 97.9 | 0.9998 |
TBP | Fw: ATATAATCCCAAGCGGTTTGCTG Rev: AAAATCAGTGCCGTGGTTCG | 66 | 79.8 | Intron-spanning | 97.4 | 0.9989 |
UBC | Fw: GGTCGCAGTTCTTGTTTGTGG Rev: TTCACGAAGATCTGCATTGTCAAG | 60 | 78.4 | Exonic | 100.7 | 0.9998 |
PPIA | Fw: TGGGTTACTTCTGAAACATCACTTGT Rev: TTGACACTTCCTGGGACTGGA | 85 | 75.1 | Exonic | 98.2 | 0.9999 |
RPL30 | Fw: TTCTCGCTAACAACTGCCCA Rev: TGCCACTGTAGTGATGGACAC | 90 | 78.4 | Intron-flanking | 95.9 | 0.9991 |
ACTB | Fw: ACAGAGCCTCGCCTTTGC Rev: CGCGGCGATATCATCATCCA | 76 | 86.9 | Intron-flanking | 95.2 | 0.9998 |
GAPDH | Fw: GAGAAGGCTGGGGCTCATTT Rev: TGATGACCCTTTTGGCTCCC | 46 | 79.4 | Intron-spanning | 97.8 | 0.9999 |
