Table 4 Ranking of candidate reference genes.

From: Identification of new reference genes with stable expression patterns for gene expression studies using human cancer and normal cell lines

Comprehensive rank

Gene symbol

GeNorm

Comparative ΔCt

NormFinder

BestKeeper

Geometric mean

Rank

M value

Rank

Mean StdDev

Rank

Stability value

Rank

Stability value

All cell lines

1

IPO8

1

0.381

3

0.555

1

0.205

3

0.336

1.73

2

PUM1

2

0.384

4

0.568

4

0.222

1

0.326

2.38

3

HNRNPL

3

0.386

1

0.538

3

0.215

6

0.353

2.71

4

SNW1

4

0.407

2

0.545

2

0.206

4

0.344

2.83

5

CNOT4

5

0.428

5

0.597

5

0.266

5

0.345

5.00

6

GAPDH

10

0.547

7

0.652

6

0.268

2

0.328

5.38

7

PCBP1

6

0.45

6

0.612

7

0.273

7

0.394

6.48

8

TBP

7

0.477

9

0.678

9

0.342

11

0.483

8.89

9

PPIA

8

0.504

8

0.657

8

0.308

10

0.471

8.46

10

RPL30

9

0.523

10

0.701

11

0.366

8

0.406

9.43

11

UBC

11

0.587

11

0.777

10

0.362

9

0.457

10.22

12

ACTB

12

0.636

12

0.911

12

0.503

12

0.679

12.00

Cancer cell lines

1

HNRNPL

1

0.333

1

0.475

1

0.160

1

0.275

1.00

2

IPO8

2

0.346

3

0.535

2

0.212

5

0.322

2.78

3

PUM1

3

0.363

4

0.539

4

0.215

4

0.32

3.72

4

SNW1

5

0.416

2

0.533

3

0.213

8

0.368

3.94

5

CNOT4

4

0.393

5

0.564

6

0.257

6

0.332

5.18

6

GAPDH

10

0.523

7

0.616

5

0.245

3

0.31

5.69

7

RPL30

7

0.467

9

0.627

11

0.321

2

0.308

6.10

8

UBC

6

0.437

6

0.599

7

0.260

7

0.355

6.48

9

PCBP1

9

0.503

8

0.623

8

0.299

9

0.394

8.49

10

TBP

8

0.487

10

0.640

10

0.312

10

0.447

9.46

11

PPIA

11

0.539

11

0.644

9

0.308

11

0.458

10.46

12

ACTB

12

0.595

12

0.904

12

0.510

12

0.716

12.00

Normal cell lines

1

IPO8

1

0.223

1

0.495

1

0.149

3

0.318

1.32

2

SNW1

2

0.236

3

0.515

2

0.187

1

0.29

1.86

3

PUM1

3

0.26

2

0.508

5

0.218

4

0.323

3.31

4

CNOT4

9

0.433

5

0.552

3

0.203

2

0.301

4.05

5

PCBP1

6

0.368

4

0.544

4

0.214

6

0.386

4.90

6

PPIA

5

0.349

7

0.570

6

0.282

7

0.422

6.19

7

HNRNPL

4

0.327

6

0.568

7

0.286

9

0.455

6.24

8

GAPDH

10

0.481

9

0.678

8

0.306

5

0.368

7.75

9

RPL30

7

0.387

8

0.632

9

0.318

8

0.437

7.97

10

TBP

8

0.407

10

0.685

10

0.374

10

0.533

9.46

11

ACTB

11

0.554

11

0.884

11

0.464

11

0.553

11.00

12

UBC

12

0.622

12

0.967

12

0.554

12

0.617

12.00

  1. The comprehensive rank is generated based on the geometric mean of ranks. The best combinations of reference genes as suggested by NormFinder are shown in bold.
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