Figure 6

Expression of NLR genes in OSP and null mutation in NLR-C. (a) Transcript accumulation of the three NB-LRR genes (NLR-A, NLR-B, and NLR-C) estimated using semi-quantitative RT-PCR. Expression of NLR-A, NLR-B, and NLR-C in leaves. Accumulation of NLR-C transcripts coincides with the presence of the fenitrothion-sensitive (S) NOG genotype (NOG, RIL25-78, − 80, and − 81), whereas no transcript was detected in BTx623 and the resistant (R) line (RIL25-79). ‘+’ and ‘−’ indicate gene expression analysis in the presence or absence of organophosphate pesticide (OP), respectively. The PP2A gene was used as an internal control. The original gel images are provided in Supplementary Information 2. (b) Schematic diagram of the NLR-C coding region and its 5’ upstream region. NLR-C consists of a single exon (top), and the closeup view of the 593-bp insertion specific to NOG is shown below. Putative transcription start site (TSS, indicated by an arrow) is located 227 bp upstream from the 3′ end of the insertion. The nucleotide sequence of the insertion is shown below the diagram, where the putative TSS determined by 5′ RACE and the TATA-box sequence are indicated. A schematic diagram of the insertion region is shown, which contains the TSS and putative TATA-box, upstream NLR-C gene in NOG genetic background.