Table 2 Contributions of various monosaccharides to relative mobility (rm).

From: Fluorescent glycan fingerprinting of SARS2 spike proteins

Glycans and their rm

Contributions of individual monosaccharides to rm

Glycans

rm

Pairs of glycans

difference by monosaccharides

Δrm

Δrm/monosaccharide

FA2G2F2

− 0.521

FA2G2F2, FA2G2

2 × Fuc

− 0.185

− 0.092/Fuc

FA2G2

− 0.336

FA2G2, FA2

2 × Gal

− 0.336

− 0.168/Gal

FA3B

− 0.240

FA3B, FA3

1 × b-GlcNAc

− 0.087

− 0.087/b-GlcNAc

FA3

− 0.154

FA3, FA2

1 × GlcNAc

− 0.154

− 0.154/GlcNAc

FA2B

− 0.092

FA2B, FA2

1 × b-GlcNAc

− 0.092

− 0.092/b-GlcNAc

FA2

0.000

    

FA2G2S(6)1

0.381

FA2G2S(6)1, A2G2S(6)1

1 × core-Fuc

− 0.119

− 0.119/core-Fuc

A2G2S(6)1

0.501

    

FA2G2S(3)2

0.894

FA2G2S(3)2, FA2G2

2 × α3-Sia

1.231

0.615/α3-Sia

FA2G2S(6)2

1.000

FA2G2S(6)2, FA2G2

2 × α6-Sia

1.336

0.676/α6-Sia

  1. The rm of each individual glycan was determined based on the gel images in Fig. 2, where the relative mobility of FA2 was arbitrarily set to 0 and that of FA2G2S(6)2 was set to 1. The contribution of a monosaccharide to the mobility was calculated based on the difference in the mobility of a pair of glycans that differed only by the monosaccharide. A minus number indicates that the monosaccharide slows down the mobility and a positive number indicates that the monosaccharide makes the mobility faster.
  2. b-GlcNAc bisecting-GlcNAc.
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