Table 1 List of the primers used in RACE PCR and real-time quantitative PCR (qPCR) in this study.
Primer | Sequence (5′–3′) | Amplicon size (bp) | R2 | Efficiency (%) | Application |
|---|---|---|---|---|---|
McDx-5’R1 | ACATCACAATAAAACCACCAACACT | 5′RACE | |||
McDx-5’R2 | TGATGCCCTGGCCTCTAAGTATTCC | 5′RACE | |||
McDx-3’F1 | GCAGACGAATGGAGTGTACAAGGCA | 3′RACE | |||
McDx-3’F2 | AGTGCTTTCCCAGAACGTTACTACG | 3′RACE | |||
McDy-3’F1 | CAGCAGCAAAACGGCTACAATCTT | 3′RACE | |||
McDy-3’F2 | GGTGAAAGAGGACCAGTAGGGTATCG | 3′RACE | |||
McDx-F | AGCTGCTCTTGACCACCGTTTATG | 142 | 0.99 | 96 | qPCR |
McDx-R | ACGTTGTGCCTTGTACACTCCATTC | qPCR | |||
McDy-F | TTCAAACAAGTATGGAGGGCGATGT | 179 | 0.99 | 98 | qPCR |
McDy-R | AACGGCGGTCACGAACAACTG | qPCR |
