Figure 4
From: Molecular principles of recruitment and dynamics of guest proteins in liquid droplets
p53 diffuses slowly in the droplets, irrespective of its oligomeric state and droplet-forming disordered domain removal. (A) Snapshot (left) and time-averaged (right) fluorescent images of Alexa488-labeled p53 tetramer in a non-labeled p53 tetramer droplet solution. In the left panel, the arrows represent the single p53 molecules. In the right panel, the typical trajectories of single molecules (red) are overlaid. Scale bar denotes 5 μm. (B) Mean square displacement (MSD) plots of Alexa488-labeled p53 mutants in non-labeled p53 tetramer droplets. p53 NCT and TC denote mutants lacking the C-terminal domain and N-terminal plus core domains, respectively, maintaining a tetramer. The straight lines show the best-fit linear functions for the MSD data. Error bars denote standard errors.
