Table 1 Enrichment indices, physical parameters, and diffusion coefficients of guest proteins in the droplets of p53 tetramer.

From: Molecular principles of recruitment and dynamics of guest proteins in liquid droplets

Guest proteins

Enrichment index

D (μm2 s−1)

Length (AA)

Total charge *

R + Y

IDPs

p53 tetramer

18.9 ± 0.8

0.031 ± 0.002

1572

384

140

p53 L344A (dimer)

14.6 ± 0.7

0.039 ± 0.002

786

192

70

p53 L344P (monomer)

1.51 ± 0.04

0.046 ± 0.004

393

96

35

p53 NCT (tetramer)

15.3 ± 0.6

0.026 ± 0.003

1452

344

136

p53 TC (tetramer)

16.0 ± 0.4

0.054 ± 0.006

408

132

32

RGG domain of LAF-1

5.4 ± 0.3

0.071 ± 0.003

168

44

35

FUS-MBP

8.5 ± 0.6

0.051 ± 0.005

934

187

95

Nhp6A

6.9 ± 0.2

0.048 ± 0.004

93

36

11

poly-Arg

10.2 ± 0.3

0.036 ± 0.003

200

200

200

poly-Asp

1.31 ± 0.02

0.052 ± 0.003

200

200

0

Folded proteins

HU (dimer)

1.63 ± 0.02

0.43 ± 0.06

184

56

8

Fis (dimer)

5.1 ± 0.2

0.93 ± 0.08

196

46

20

CRP-GFP (dimer)

5.5 ± 0.2

0.19 ± 0.02

912

224

68

Fpg

6.9 ± 0.2

1.31 ± 0.09

272

81

26

dCas9

15.6 ± 0.5

0.070 ± 0.004

1371

433

132

dCas9-MBP

11.4 ± 0.4

0.048 ± 0.003

1779

528

153

GFP

2.4 ± 0.1

1.67 ± 0.08

246

62

17

Venus

1.84 ± 0.06

 

246

61

18

  1. *The total charge number was calculated as the number of R, K, D, and E.