Table 1 Enrichment indices, physical parameters, and diffusion coefficients of guest proteins in the droplets of p53 tetramer.
From: Molecular principles of recruitment and dynamics of guest proteins in liquid droplets
Guest proteins | Enrichment index | D (μm2 s−1) | Length (AA) | Total charge * | R + Y |
|---|---|---|---|---|---|
IDPs | |||||
p53 tetramer | 18.9 ± 0.8 | 0.031 ± 0.002 | 1572 | 384 | 140 |
p53 L344A (dimer) | 14.6 ± 0.7 | 0.039 ± 0.002 | 786 | 192 | 70 |
p53 L344P (monomer) | 1.51 ± 0.04 | 0.046 ± 0.004 | 393 | 96 | 35 |
p53 NCT (tetramer) | 15.3 ± 0.6 | 0.026 ± 0.003 | 1452 | 344 | 136 |
p53 TC (tetramer) | 16.0 ± 0.4 | 0.054 ± 0.006 | 408 | 132 | 32 |
RGG domain of LAF-1 | 5.4 ± 0.3 | 0.071 ± 0.003 | 168 | 44 | 35 |
FUS-MBP | 8.5 ± 0.6 | 0.051 ± 0.005 | 934 | 187 | 95 |
Nhp6A | 6.9 ± 0.2 | 0.048 ± 0.004 | 93 | 36 | 11 |
poly-Arg | 10.2 ± 0.3 | 0.036 ± 0.003 | 200 | 200 | 200 |
poly-Asp | 1.31 ± 0.02 | 0.052 ± 0.003 | 200 | 200 | 0 |
Folded proteins | |||||
HU (dimer) | 1.63 ± 0.02 | 0.43 ± 0.06 | 184 | 56 | 8 |
Fis (dimer) | 5.1 ± 0.2 | 0.93 ± 0.08 | 196 | 46 | 20 |
CRP-GFP (dimer) | 5.5 ± 0.2 | 0.19 ± 0.02 | 912 | 224 | 68 |
Fpg | 6.9 ± 0.2 | 1.31 ± 0.09 | 272 | 81 | 26 |
dCas9 | 15.6 ± 0.5 | 0.070 ± 0.004 | 1371 | 433 | 132 |
dCas9-MBP | 11.4 ± 0.4 | 0.048 ± 0.003 | 1779 | 528 | 153 |
GFP | 2.4 ± 0.1 | 1.67 ± 0.08 | 246 | 62 | 17 |
Venus | 1.84 ± 0.06 | 246 | 61 | 18 | |