Figure 3 | Scientific Reports

Figure 3

From: The extracellular matrix glycoprotein ADAMTSL2 is increased in heart failure and inhibits TGFβ signalling in cardiac fibroblasts

Figure 3

ADAMTSL2 alters expression of ECM and ECM-associated genes in human cardiac fibroblasts. Human foetal cardiac fibroblasts were cultured for four or seven days (see Supplementary Fig. S3a), forming an immature or mature extracellular matrix (ECM), and transduced with ADAMTSL2 (L2) or vehicle control (Veh) adenoviruses on day one or four, respectively. Data represent experiments from three different cell passages. (a) Incorporation of [3H]-proline representing total collagen synthesis in L2 vs. Veh (n = 36). (b, c) FBN1, FBN2 and ELN mRNA levels in L2 vs. Veh (n = 9–15). Gene expression was normalized to RPL4 (b, c). Data are presented as mean ± min/max values and statistical analysis was performed using the Student t-test vs. respective controls. (d) Gene expression array of 84 ECM and adhesion molecule genes showing 50 differentially expressed genes, ± 0.5-fold in L2 vs. Veh (pools of n = 3–6). Gene expression was normalized to GAPDH and Veh and presented as –ΔΔCT values.

Back to article page