Figure 3 | Scientific Reports

Figure 3

From: Soft surfaces promote astrocytic differentiation of mouse embryonic neural stem cells via dephosphorylation of MRLC in the absence of serum

Figure 3

PP-MRLC of mouse embryonic neural stem cells is decreased by culturing on soft surfaces in the absence of serum. (a) Mouse embryonic NSCs were cultured on three types of plate (1 kPa plates, 12 kPa plates, and commonly used plastic plates [2.8 GPa]) for 3 days in serum-free condition. Protein expression of PP-MRLC, total MRLC, and GAPDH (for loading control) on each plate type was detected by western blotting analysis. The expression levels of (b) PP-MRLC and (c) total MRLC proteins were normalized relative to that of GAPDH protein. Error bars represent the standard deviation; *p < 0.05, ***p < 0.005 (Student’s t test with Bonferroni correction). NSCs neural stem cells, PP-MRLC di-phosphorylated myosin regulatory light chain.

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