Figure 5 | Scientific Reports

Figure 5

From: Soft surfaces promote astrocytic differentiation of mouse embryonic neural stem cells via dephosphorylation of MRLC in the absence of serum

Figure 5

Treatment with ROCK inhibitor increases mRNA expression of astrocyte markers after 3 days of differentiation of mouse embryonic neural stem cells in the absence of serum. (a) Mouse embryonic NSCs were cultured on commonly used plastic plates for 3 days without (control) or with 10 μM Y27632 (Y27632) in serum-free condition. mRNA expression of GFAP, S100B and GAPDH on each plate type was detected by qPCR analysis. The expression levels of (a) GFAP and (b) S100B were normalized relative to that of GAPDH. Error bars represent the standard deviation; *p < 0.05, ***p < 0.005 (Student’s t test). ROCK Rho-associated kinase, NSCs neural stem cells, GFAP glial fibrillary acidic protein, S100B S100 calcium binding protein B, GAPDH glyceraldehyde 3-phosphate dehydrogenase, qPCR quantitative polymerase chain reaction.

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