Table 1 Commercially available reagents for fluorescent nanoparticle probes.
From: Modular fluorescent nanoparticle DNA probes for detection of peptides and proteins
Component of fluorescent nanoparticle probe | Vendor | Catalog No | Product name |
|---|---|---|---|
Nanoparticle label | Thermo Fisher Scientific | F8795 | FluoSpheres Carboxylate-Modified Microspheres, 0.04 µm, yellow-green fluorescent (505/515), 5% solids, azide free |
Thermo Fisher Scientific | F8811 | FluoSpheres Carboxylate-Modified Microspheres, 0.2 µm, yellow-green fluorescent (505/515), 5% solids | |
Thermo Fisher Scientific | F8807 | FluoSpheres Carboxylate-Modified Microspheres, 0.2 µm, dark-red fluorescent (660/680), 5% solids | |
Thermo Fisher Scientific | Q21321MP | Qdot 655 ITK Carboxyl Quantum Dots | |
Passivation and Functionalization | Creative PEGWorks | PHB-1882 | Amine-PEG-azide, MW 2 kDa |
Creative PEGWorks | PLS-269 | mPEG-Amine, MW 2 kDa | |
Conjugation annealing handle | Integrated DNA Technologies | Custom sequence; Table 3 | < custom_sequence > /iSp9//3DBCON/, HPLC purification |
DNA aptamer probe | Integrated DNA Technologies | Custom sequence; Table 3 | 20 nmole Ultramer |
