Figure 1 | Scientific Reports

Figure 1

From: Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations

Figure 1

Brain microvascular endothelial cells adhere selectively to the RGD-patterned lines. First row: Lines of fluorescent 5-FAM-GCGYRGDSPG peptide patterned on HA-am hydrogels (green). Second row: b.End3 cells seeded on the lines stained for F-actin (red) and nucleus (blue). Third row: b.End3 cells seeded on the lines stained for zonula occludens-1 (ZO-1) (yellow) and nucleus (blue). Forth row: Merged images showing the cells on the micropatterned lines illustrating that the cells are adhering only to the peptide-patterned lines. Scale bars correspond to 100 µm in all images.

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