Figure 2 | Scientific Reports

Figure 2

From: Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations

Figure 2

Schematic representing the data analysis. The nucleus, cell, or F-actin orientation with respect to the underlying peptide pattern and the nucleus or cell elongation was analyzed using Cell Profiler. (A) The cell, nuclei and F-actin were segmented from the images and fitted to an ellipse. The major and minor axis of the ellipse representation of the objects are identified. The orientation is determined by the angle between the major axis of the object and the underlying RGD pattern direction. (B) The nucleus or cell eccentricity indicates the elongation, where 1 is a line and 0 is a perfectly round circle.

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