Figure 3
Brain microvascular endothelial cells align with the direction of the peptide pattern and alignment increases as the line width decreases. Box plots of actin orientation, nuclei orientation and cell orientation for the different line widths 10 µm, 25 µm, 50 µm, 75 µm, 100 µm, as well as a non-patterned (NP) control where the whole surface was covered with RGD. For the statistical analysis 400 nuclei and cells and 4000 actin fibres were used for each condition. The statistics included in figure 3 are summarized in Supplementary Tables S1, S2, S3, and *denotes p ≤ 0.05, **denotes p ≤ 0.01, and ***denotes p ≤ 0.001.
