Figure 6 | Scientific Reports

Figure 6

From: Contamination-resistant, rapid emulsion-based isothermal nucleic acid amplification with Mie-scatter inspired light scatter analysis for bacterial identification

Figure 6

Sensitivity and specificity in comparison to conventional LAMP. (A) Average 30° light scatter intensity profiles for 103 CFU/μL and NC (negative control) samples. (B) Comparison of light scatter intensity values at 5 min between initial target bacterial concentrations. (C) Gel image showing amplification of both positive (left lanes) and NC (right lanes) samples after conventional amplification for 30 min. (D) Table detailing sensitivity and specificity of both conventional amplifications combined with gel electrophoresis and emulsion LAMP.

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