Figure 1
From: Reducing soft-tissue shrinkage artefacts caused by staining with Lugol’s solution
adapted from Organ specific shrinkage in iodine stained human fetuses13. White arrows point to the empty space between skull and brain and liver and body cavity, which is reminiscent of soft-tissue shrinkage. B = Brain, H = Heart, Li = Liver, Lu = Lung. Scale bar represents 10 mm.
Micro-CT images of a human fetus at 19 weeks of gestation (total length = 24 cm). The fetus was stained with 3.75% Lugol’s solution for 26 days in total. The fetus was scanned on a GE Phoenix v|tome|x scanner (General Electric, Wunstorf, Germany) with an isotropic resolution of 50 µm. (A) Transversal section at the level of the heart, (B) mid coronal section and (C) mid sagittal section. Volume analyses showed that the staining caused extensive shrinkage: 9% in total fetal volume, 24% in lung and liver volume, 31% in kidney volume and 33% in brain volume Images
