Table 1 Method characteristics for simultaneous analysis of urinary total NNAL, NNN and cotinine.

From: Simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, N′-nitrosonornicotine, and cotinine by liquid chromatography-tandem mass-spectrometry

Characteristic

Biomarkera

NNAL

NNN

Cotinine

Accuracyb, %

110.9 (102.5–115.3)

89.2 (85.1–96.4)

107.2 (103.5–113.3)

Precisionb, %

2.9 (0.5–7.2)

4.9 (1.5–13.8)

1.9 (0.8–2.6)

Intra-day variation, % (N = 6)

10.3

7.0

8.6

Inter-day variation, % (N = 12)

10.2

11.2

9.5

  1. aBiomarker abbreviations: NNAL, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol; NNN, N′-nitrosonornicotine.
  2. bAccuracy and precision were determined in pooled urine from non-users of tobacco to which biomarkers were added in the ranges from 0.1 to 23.9 pmol/mL for NNAL, 0.1 to 5.6 pmol/mL for NNN, and 0.6 to 84.7 nmol/mL for cotinine. The numbers shown are accuracy and precision averages for all levels of addition and minimum–maximum ranges (in parentheses). For more detail on method characterization see “Methods” section.
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