Figure 3
From: Generation of knock-in lampreys by CRISPR-Cas9-mediated genome engineering
MA2:EGFP knock-in lampreys. (A) A MA2:EGFP knock-in lamprey showing EGFP expression in the head region at stage 30, in lateral view. The head region is magnified in (A’). The asterisk (*) indicates the eyeball. EGFP is expressed both somatic and pharyngeal muscles (m.). In the york, some non-specific signals (by unintegrated plasmids) were observed (arrows). A, D, P, V indicate anterior, dorsal, posterior, and ventral, respectively. Scale bar: 500 μm. (B) A MA2:EGFP knock-in lamprey showing EGFP expression in the trunk region at stage 30, in lateral view. A part of the trunk region is magnified in (B’). Both EGFP-positive and EGFP-negative somatic muscle cells are observed in the same somite. In the york, some non-specific signals (by unintegrated plasmids) were observed (arrows). A, D, P, V indicate anterior, dorsal, posterior, and ventral, respectively. Scale bar: 500 μm.
