Figure 5 | Scientific Reports

Figure 5

From: Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides

Figure 5

(A) Distribution of the products formed by BRS-B Δ2 enzyme and its mutants, and determined from HPLC–UV analyses of 16 h reaction mixture with 50 mM ABC’D’, 1 M sucrose at pH 5.75 and variable amounts of purified enzymes in 50 µL reaction volume. Mass Spectrometry revealed a mass increase of 162 g mol−1 compared to ABC’D’, corresponding to mono-glucosylated tetrasaccharides. M14 and M18 did not produce any pentasaccharide product (not shown). (B) An enlarged view of the products (notably novel products P2″ and P3’) formed by M35 and M40 is shown on the right panel.

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