Figure 6 | Scientific Reports

Figure 6

From: Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides

Figure 6

UHPLC-ESI–MS/MS spectrum of the pentasacharide M40-4 isolated as [M-H]− at m/z 1038.21 at tR = 22.86 min in samples from M40 validated as being substituted at OH-3B (P3’). The blue area of the spectrum is enlarged by a factor of 4 in the intensity axis. Annotations in red correspond to intact product ions. Annotation in blue correspond to product ions with a loss of CHCl3. Annotations in purple correspond to product ions with a loss of COCHCl. Annotation in orange correspond to product ions with a loss of CHCl3 + COHCl3. All these labile function losses are in agreement with the structure. Red * indicate consecutive fragmentations, ▽ indicate H2O loss, ◊ indicate HCl loss. For clarity, only one fragment per pair (B, C and Y, Z) was reported on the annotated structure.

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