Figure 8 | Scientific Reports

Figure 8

From: Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides

Figure 8

Diversity of pentasaccharides obtained with the BRS-B Δ2 mutants. The various products are shown on the basis of the glucosylated subunit (A, B or D’). P1 ABC’[E(1 → 6)]D’, characterized in ref 24. P2 [E(1 → 3)]ABC’D’ and P2’ [E(1 → 4)]ABC’D’ characterized in ref 21. P3, P3’ and P2″ were characterized in this study to be A[E(1 → 4)]BC’D’ and A[E(1 → 3)]BC’D’, and E[(1 → 2)]ABC’D’, respectively. P3 was characterized by NMR spectroscopy and P3’ and P2″ by MS/MS, respectively. ClAc: chloroacetyl. Drawing of chemical structures done using CHEMDRAW (PerkinElmer).

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