Table 1 Animal manure samples and sources.

From: Applying PICRUSt and 16S rRNA functional characterisation to predicting co-digestion strategies of various animal manures for biogas production

Sample

Sources

Sample ID

Location

Description of location and handling of livestock

Cow dung

Boerdery farm produce

C1

 − 26.21779, 27.6414784

A farm producing animal feeds as well as rearing cattle fed on grains and concentrates in Randfontein, Gauteng

Kates farm

C2

 − 26.0990952, 27.9103166

A bed and breakfast farm facility in the outskirts of Johannesburg, Gauteng serving beef and chicken from livestock locally reared in a free-range feeding scheme

Bosheuvel country estates

C3

 − 26.0249182, 27.8929324

A farm in the Muldersdrift area of Johannesburg with an on-site vintage hotel, restaurant, event venues and conference rooms serving locally reared Pinzgauer cattle, pigs, chickens, and sheep fed mostly on mixed vegetables and tubbers

Horse manure

Earth centre

H1

 − 26.080926, 27.8747353

A non-profit company situated in Ruimsig, Gauteng specialising in Equine Assisted Programmes for children with disabilities.The horses are fed with a wide range of feed concentrates and probiotics

Harveston stables

H2

 − 26.0990952, 27.9103167

A yard in Honeydew, Gauteng offering horse riding lessons, stabling, kids pony parties, picnics and pony rides. The horses are fed mainly on feed concentrates

Barent horse stables

H3

 − 26.21779, 27.6414783

A plot rearing horses for family leisure in Randfontein, Gauteng. The horses are fed mostly on lucerne

Pig manure

Bosheuvel country estates

P1

 − 26.0249182, 27.8929324

A farm in the Muldersdrift area of Johannesburg with an on-site vintage hotel, restaurant, event venues and conference rooms serving locally reared Pinzgauer cattle, pigs, chickens, and sheep fed mostly on mixed vegetables and tubbers

Bosheuvel country estates

P2

 − 26.0249182, 27.8929324

Country portion farm

P3

 − 26.224517, 27.6325603

A farm in Randfontein producing vegetables, poultry, and pork products to supply local restaurants and consumers. The poultry and pigs are fed on corn and sorghum and concentrates