Table 1 Animal manure samples and sources.
Sample | Sources | Sample ID | Location | Description of location and handling of livestock |
|---|---|---|---|---|
Cow dung | Boerdery farm produce | C1 |  − 26.21779, 27.6414784 | A farm producing animal feeds as well as rearing cattle fed on grains and concentrates in Randfontein, Gauteng |
Kates farm | C2 |  − 26.0990952, 27.9103166 | A bed and breakfast farm facility in the outskirts of Johannesburg, Gauteng serving beef and chicken from livestock locally reared in a free-range feeding scheme | |
Bosheuvel country estates | C3 |  − 26.0249182, 27.8929324 | A farm in the Muldersdrift area of Johannesburg with an on-site vintage hotel, restaurant, event venues and conference rooms serving locally reared Pinzgauer cattle, pigs, chickens, and sheep fed mostly on mixed vegetables and tubbers | |
Horse manure | Earth centre | H1 |  − 26.080926, 27.8747353 | A non-profit company situated in Ruimsig, Gauteng specialising in Equine Assisted Programmes for children with disabilities.The horses are fed with a wide range of feed concentrates and probiotics |
Harveston stables | H2 |  − 26.0990952, 27.9103167 | A yard in Honeydew, Gauteng offering horse riding lessons, stabling, kids pony parties, picnics and pony rides. The horses are fed mainly on feed concentrates | |
Barent horse stables | H3 |  − 26.21779, 27.6414783 | A plot rearing horses for family leisure in Randfontein, Gauteng. The horses are fed mostly on lucerne | |
Pig manure | Bosheuvel country estates | P1 |  − 26.0249182, 27.8929324 | A farm in the Muldersdrift area of Johannesburg with an on-site vintage hotel, restaurant, event venues and conference rooms serving locally reared Pinzgauer cattle, pigs, chickens, and sheep fed mostly on mixed vegetables and tubbers |
Bosheuvel country estates | P2 |  − 26.0249182, 27.8929324 | ||
Country portion farm | P3 |  − 26.224517, 27.6325603 | A farm in Randfontein producing vegetables, poultry, and pork products to supply local restaurants and consumers. The poultry and pigs are fed on corn and sorghum and concentrates |