Figure 2 | Scientific Reports

Figure 2

From: Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants

Figure 2

Dothistroma septosporum NZE10 candidate effectors (DsCEs) induce cell death responses in non-host plants. Genes encoding DsCEs, with a PR1α signal peptide for secretion to the plant apoplast, were introduced into Nicotiana tabacum (Nt; top row of images) and N. benthamiana (Nb; middle row of images) by agro-infiltration. Cell death responses were observed at 6 days post-infiltration. Representative images are shown, along with the percentages of all infiltrations where cell death occurred (n = 12–24 infiltration zones, from at least three independent experiments). Ds43416 is included as a representative of DsCEs that did not elicit a cell death response in either of the plant species tested. The bottom row of images shows the results of agro-infiltration of DsCE proteins lacking a PR1α signal peptide (ΔSP; no secretion into the apoplast) in N. tabacum (DsEcp2-1, Ds70694, Ds70057, Ds71487 and Ds131885) or N. benthamiana (Ds74283). INF1, Phytophthora infestans elicitin positive control; EV, empty vector negative control.

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