Figure 3 | Scientific Reports

Figure 3

From: Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy

Figure 3

After transfection with pGC-siE6A or pGC-siE7 plasmid, the expression of HPV-E6/E7 protein was decreased in Siha cells. (A) The map of pGC-siRNA. (B) Semi-quantitative RT-PCR analysis of HPV16-E6/E7 mRNA in Siha cells transfected with pGC-siRNA plasmid. (C,D) Western blotting analysis of HPV16-E6 and HPV16-E7 protein in Siha cells transfected with pGC-siRNA plasmid. (E) Transfection efficiency determined by fluorescence microscope. (F,G) Immunofluorescence analysis for HPV16-E6/E7 protein in Siha cells transfected with pGC-siRNA plasmids (× 600).

Back to article page