Figure 4

The pCG-siE6A and pCG-siE7 can promote the apoptosis of Siha cells. (A) Survival ratios for Siha cells transfected with pGC-siRNA plasmids (n = 3). **P < 0.01, vs the control group. (B,C) FCAS analysis for apoptosis rates of Siha cells transfected with pGC-siRNA plasmids. *P < 0.05, **P < 0.01, vs the two control groups. (D) FCAS analysis for cell cycle of Siha transfected with pGC-siRNA plasmids. **P < 0.01, vs the Scramble group. (E) TUNEL analysis for Siha cells transfected with pGC-siRNA plasmids (× 400). (F) Semi-quantitative RT-PCR analysis of mRNA in Siha cells transfected with pGC-siRNA plasmids, **P < 0.01, vs the two control groups. (G,H) Western blotting analysis of related protein in Siha cells transfected with pGC-siRNA plasmids, *P < 0.05, vs the two control groups.