Figure 5
From: Compartment specific regulation of sleep by mushroom body requires GABA and dopaminergic signaling
Pan-neuronal knockdown of dopamine receptors DopR1 and DopR2 increases sleep amount without altering waking activity. (a) Total sleep or sleep duration over 24 h in flies expressing validated UAS-RNAi lines targeting dopamine receptors specifically: DopR1 (31765, and 62193-red), DopR2 (65997-blue), Dop2R (2 lines: 26001 and 50621-yellow) and DopEcR (31981-black) pan-neuronally using nsyb-GAL4. Sleep data represents 2-day average (Day 3 and 4) at 24οC after 2-day entrainment. nsyb-GAL4/+ flies (grey bars) were used as a negative control. (b) Representative sleep profile of RNAi mediated depletion of DopR1 (red) and DopR2 (blue) receptors in nsyb-GAL4 and control nsyb/+ (grey). ZT indicates zeitgeber time where ZT 0: lights on and ZT 12: lights off. (c,d) Average bout length and number of bouts. (e) Activity or average beam crossings/waking minute indicative of locomotor activity of all tested genotypes. (f) Sleep latency or time to sleep was calculated as the time gap between lights off and first sleep bout. Latency was reduced in nsyb/DopR1-RNAi (31765) and nsyb/DopR2-RNAi 65997) flies as compared to nsyb/+. For each of the experimental groups tested we had 88–98 flies which represented 4 independent experimental trials, sample included nsyb/+ (95), nsyb/31765 (96), nsyb/62193 (98), nsyb/65997 (93), nsyb/26001 (96), nsyb/50621 (96), and nsyb/31981 (88). Data represents mean and SEM, * indicates p < 0.05, ** indicates p < 0.001 and *** indicates p < 0.0001. Statistical analysis was one-way ANOVA and Dunnett’s paired comparison with control for (a,e,f) and Kruskal–Wallis non-parametric one-way ANOVA and Dunn’s post-hoc correction for (c,d).
