Figure 6
From: Compartment specific regulation of sleep by mushroom body requires GABA and dopaminergic signaling
Knockdown of DopR1 in MB-KCs and MBONs of the sleep-regulating γ5 and β′2 compartment increased sleep. (a) Schematic of MB wake-promoting γ5, γ4 and β′2 compartments that represents the interactions between KCs, MBONs and PAM-DANs. (b,c) Total Sleep during a 24-h period in flies expressing UAS-DopR1 RNAi (31765 and 62193) in MB-KCs (MB010B, MB107B: red), MBONs (MB011B, MB 298B: blue) and enhancerless/empty split-GAL4 pBD (control: grey). (d,e) Average bout length and number of bouts in flies expressing UAS-DopR1 RNAi (31765) in MB-KCs (MB010B, MB107B: red), MBONs (MB011B, MB298B: blue) and enhancerless/empty split-GAL4 pBD (control: grey). (f) Activity or average beam crossings/waking minute indicative of locomotor activity of flies expressing UAS-DopR1 (31765). (g,h) Average bout length and number of bouts in flies expressing UAS-DopR1 RNAi (62193) in MB-KCs (MB010B, MB107B: red), MBONs (MB011B, MB298B: blue) and enhancerless/empty split-GAL4 pBD (control: grey). (i) Activity or average beam crossings/waking minute indicative of locomotor activity of flies expressing UAS-DopR1 RNAi (62193). For each of the experimental groups tested we had 37–48 flies which represented 2 independent experimental trials, sample included 31765/pBD (48), 31765/MB010B (40), 31765/MB107B (38), 31765/MB011B (38), 31765/MB298B (37), 62193/pBD (47), 62193/MB010B (39), 62193/MB107B (44), 62193/MB011B (43), and 62193/MB298B (47). Data represents mean and SEM, * indicates p < 0.05, ** indicates p < 0.001 and *** indicates p < 0.0001. Statistical analysis was one-way ANOVA and Dunnett’s paired comparison with control for (b,c,f,i) and Kruskal–Wallis non-parametric one-way ANOVA and Dunn’s post-hoc correction for (d,e,g,h).
