Figure 8

Wakefulness induced by PAM γ5 dopamine activation requires both DopR1 and DopR2 function. (a) 3-day Sleep profile of flies expressing temperature sensitive cation channel dTrpA1 in PAM γ5 DANs (MB054B) in the wild type, DopR1 and DopR2 hypomorph backgrounds. Day 1 represents baseline at 21 °C, Day 2 represent activation at 29 °C and Day 3 represents recovery at 21 °C. (b–d) Total sleep during Day 1 (baseline, 21 °C), Day 2 (activation, 29 °C) and Day 3 (recovery, 21 °C), of flies expressing dTrpA1 in PAM γ5 in positive control (w¹¹¹⁸) and receptor hypomorph background (DopR1^attp and DopR2^attp). (e,f) Average sleep bout duration and number of bouts on Day 2 (activation, 29 °C) of flies expressing dTrpA1 in PAM γ5 in positive control (w¹¹¹⁸) and receptor hypomorph background (DopR1^attp and DopR2^attp). (g) Activity or average beam crossings/waking minute, a measure of locomotor activity for all tested genotypes. For each of the experimental groups tested we had 59–61 flies which represented 2 independent experimental trials, sample included MB054B > dTrpA1 (59), MB054B > dTrpA1 > DopR1^attp (59) and MB054B > dTrpA1 > DopR2^attp (61). Data represents mean and SEM, * indicates p < 0.05, ** indicates p < 0.001 and *** indicates p < 0.0001. Statistical analysis was one-way ANOVA and Dunnett’s paired comparison with control for (b,c,d,g) and Kruskal–Wallis non-parametric one-way ANOVA and Dunn’s post-hoc correction for (e,f).