Table 2 Oligonucleotide primers and probes for common SARS-CoV-2 S gene and B.1.1.7 primer/probe sets.
From: Surveillance of SARS-CoV-2 lineage B.1.1.7 in Slovakia using a novel, multiplexed RT-qPCR assay
Oligonucleotide | Sequence | Tm (°C) | Secondary structure potential (kcal/mol) |
|---|---|---|---|
SARS-CoV-2 S gene—F1 | TCTtTtCCAATGTTACTTGGTTC | 54.3 | − 1.52 |
SARS-CoV-2 S gene—R1 | AGTAGGGACTGGGTCTTCGAATCT | 58.9 | − 0.94 |
B.1.1.7—F3 | GTtACTtGGTTCCATGCTATCTC | 56.8 | 1.11 |
B.1.1.7—R36 | CAACtTTtGTTGTTTTTGtGGTAAGC | 58.2 | − 2.26 |
SARS-CoV-2 S gene/B.1.1.7—P3 | AGAGGTTTGATAACCCTGtCCtACCA | 61.9 | − 1.97 |
SARS-CoV-2 S gene/B.1.1.7—P4 | TtTGCTTCCACTGAGAAGTCtAACAT | 59.0 | − 1.48 |
