Table 3 Clinical performance of SARS-CoV-2 S gene and B.1.1.7 primer/probes sets.
From: Surveillance of SARS-CoV-2 lineage B.1.1.7 in Slovakia using a novel, multiplexed RT-qPCR assay
Analysed samples | Sequencing | SARS-CoV-2 S gene | B.1.1.7 (ΔH69/ΔV70 + ΔY144) | ΔH69/ΔV70 only | Other lineage of SARS-CoV-2 | Inconclusive | RNase P |
|---|---|---|---|---|---|---|---|
B.1.1.7 (ΔH69/ΔV70 + ΔY144) | 67 | 67 | 65 | 0 | 1 | 1 | 67 |
ΔH69/ΔV70 only | 24 | 24 | 0 | 20 | 3 | 1 | 24 |
Other lineages of SARS-CoV-2 | 15 | 15 | 0 | 0 | 15 | 0 | 15 |
SARS-CoV-2 negative | 0 | 0 | 0 | 0 | 0 | 0 | 94 |
Total | 106 | 106 | 65 | 20 | 19 | 2 | 200 |
B.1.1.7 statistics | Value (95% CI) | ΔH69/ΔV70 only statistics | Value (95% CI) | ||||
|---|---|---|---|---|---|---|---|
Sensitivity | 97.01% (89.63–99.64%) | Sensitivity | 83.33% (62.62–95.26%) | ||||
Specificity | 100.00% (90.97–100.00%) | Specificity | 100.00% (95.60–100.00%) | ||||
Negative Likelihood Ratio | 0.03 (0.03–0.12) | Negative likelihood ratio | 0.17 (0.07–0.41) | ||||
Estimated disease prevalence | 25.00% | Estimated disease prevalence | 50.00% | ||||
Positive Predictive Value | 100.00% | Positive predictive value | 100.00% | ||||
Negative Predictive Value | 99.01% (96.25–99.75%) | Negative predictive value | 85.71% (71.04–93.62%) | ||||
Accuracy | 99.25% (95.19–99.99%) | Accuracy | 91.67% (84.69–96.15%) |
