Table 1 Sensitivity of duplex RT-RAA assays using cultured GETV RNA and plasmid.
Serially diluted GETV culture medium (TCID50/reaction) | Serially diluted GETV plasmid (copies/reaction) | No. replicates tested | No. detection | Detection rate (%) |
|---|---|---|---|---|
– | 8 × 105 | 8 | 8 | 100 |
– | 8 × 104 | 8 | 8 | 100 |
2 × 104 | 8 × 103 | 8 | 8 | 100 |
2 × 103 | 8 × 102 | 8 | 8 | 100 |
2 × 102 | 8 × 101 | 8 | 8 | 100 |
2 × 101 | 8 × 100 | 8 | 8 | 100 |
2 × 100 | 8 × 10−1 | 8 | 0 | 0 |
