Figure 2 | Scientific Reports

Figure 2

From: A gain and dynamic range independent index to quantify spillover spread to aid panel design in flow cytometry

Figure 2

Effect of PMT voltage on ISS: In (A), the primary detector (V605) voltage was changed from 600 to 400 V in steps of 100 V. This did not change the rSD and SQI values measuring the spread in the secondary detector (V677). However, the ISS values changed noticeably. In (A–C), voltages of both detectors were changed simultaneously to 600 V, 500 V or 400 V respectively. The SQI values remained the same for both detectors in all cases, but the ISS values decreased considerably. In (D) only voltage of V677 was lowered which resulted in a huge increase of ISS but SQI remain same.

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