Figure 5
From: A neutralizing IL-11 antibody reduces vessel hyperplasia in a mouse carotid artery wire injury model
The effect of anti-IL-11 antibody (X203) treatment on injury-related plaque VSMC accumulation. (A) SMA+ VSMCs per injury-related plaque (N = 11/group, One-way ANOVA, Tukey’s multiple comparison test, Values ± SD). (B) SMA+ VSMCs percent of all cells (N = 11/group, One-way ANOVA, Tukey’s multiple comparison test, Values ± SD). (C) SM22α expression in VSMCs in the injury-related plaque (N = 11/group, One-way ANOVA, Tukey’s multiple comparison test, Values ± SD). (D) Representative images of SMA immunofluorescence staining (red) and corresponding DAPI staining (blue, insets) in left, injured carotid arteries (scale bar 100 µm). Transparent yellow-lines were traced to delineate the injury-related plaque area used for quantifications. (E) Representative images of SM22α (green) and SMA (red) immunofluorescence co-staining (yellow, scale bar 50 μm). Transparent blue-lines were traced to delineate the injury-related plaque area used for quantifications. (F) Representative images of SMA immunofluorescence staining (red) and corresponding DAPI staining (blue, insets) in right, uninjured carotid arteries (scale bar 100 µm).
