Figure 3 | Scientific Reports

Figure 3

From: Mutant Gossypium universal stress protein-2 (GUSP-2) gene confers resistance to various abiotic stresses in E. coli BL-21 and CIM-496-Gossypium hirsutum

Figure 3

Spot assay of E. coli-BL21-uspABC. Transformed BL21-usp-ABC cells with wild type (W-usp-2) and mutant (M1-usp-2, M2-usp-2, M3-usp-2) genes were spotted on LB plates supplemented with salt (800 mM NaCl), PEG (8%). For heat stress, after 2 h of induction (IPTG) 1 ml culture was inoculated in 10 ml media (LB) and incubated at elevated temperatures (46 °C). Samples were removed after 8 h and spotted on respective plates. For cold stress, spotted plates were removed from 4 °C after 10d and photos were taken after incubation at 37 °C.

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