Table 2 Thermodynamic parameters of the CGH-1_248–420 (or its mutants) bound to its ligand CePATR-1_30–67 by ITC assays.

CGH-1_248-420^WT

PATR1_30-67^WT

2.13 ± 0.38

0.88

− 15.8 ± 0.62

8.16

CGH-1_248-420^F261A

PATR1_30-67^WT

6.62 ± 1.05

0.76

− 13.0 ± 0.70

6.08

CGH-1_248-420^V262A

PATR1_30-67^WT

4.94 ± 0.79

0.84

− 17.3 ± 0.82

10.1

CGH-1_248-420^Q266A

PATR1_30-67^WT

2.97 ± 0.29

0.86

− 16.6 ± 0.41

9.17

CGH-1_248-420^H269A

PATR1_30-67^WT

2.67 ± 0.44

0.94

− 15.4 ± 0.67

7.94

CGH-1_248-420^K277A

PATR1_30-67^WT

4.43 ± 0.42

1.07

− 17.2 ± 0.57

10.0

CGH-1_248-420^K277E

PATR1_30-67^WT

8.29 ± 0.99

1.07

− 19.6 ± 0.95

12.7

CGH-1_248-420^F355A

PATR1_30-67^WT

–

–

–

–

CGH-1_248-420^Y386A

PATR1_30-67^WT

4.19 ± 0.58

0.85

− 14.2 ± 0.56

6.95

CGH-1_248-420^R393A

PATR1_30-67^WT

4.22 ± 0.62

0.80

− 16.6 ± 0.69

9.42

CGH-1_248-420^E396A

PATR1_30-67^WT

2.96 ± 0.34

0.93

− 16.3 ± 0.47

8.89

CGH-1_248-420^4A

PATR1_30-67^WT

2.06 ± 0.27

0.79

− 15.4 ± 0.44

7.76