Table 2 Effect of the culture system, immersion frequency, and volume of culture medium on rooting, and shoot elongation.

Semi solid culture

94.3 ± 1.5^c

5.65 ± 0.7^a

9.65 ± 1.0^d

09.25 ± 0.2^d

TIB-Immersion frequencies (2-min immersion)¹

4 h

97.6 ± 1.7^ab

6.0 ± 1.0^a

10.3 ± 1.0^cd

11.1 ± 0.6^c

6 h

98.6 ± 1.0^a

6.5 ± 0.7^a

12.0 ± 1.1^b

16.4 ± 0.9^a

12 h

96.1 ± 2.0^abc

6.3 ± 0.8^a

14.4 ± 0.8^a

17.8 ± 1.9^a

Volume of culture medium (ml)²

100

95.3 ± 2.7^bc

5.5 ± 0.9^a

12.7 ± 1.5^b

14.5 ± 1.8^b

250

98.6 ± 1.1^a

6.3 ± 0.4^a

11.5 ± 1.2^bc

17.5 ± 1.4^a

500

96.4 ± 2.2^abc

6.0 ± 1.1^a

12.8 ± 1.4^b

17.7 ± 1.7^a