Table 3 List of ISSR primers used for assessment of genetic fidelity.
Primer name | Sequence 5′ to 3′ | Annealing temperature | Total number of bands—polymorphic bands—percent polymorphic bands | |
|---|---|---|---|---|
Semisolid | TIB | |||
UBC807 | AGAGAGAGAGAGAGAGT | 46.8 | 12-0-0 | 12-0-0 |
UBC808 | AGAGAGAGAGAGAGAGC | 50.6 | 8-0-0 | 8-0-0 |
UBC810 | GAGAGAGAGAGAGAGAT | 50.4 | 6-0-0 | 6-0-0 |
UBC811 | GAGAGAGAGAGAGAGAC | 46.0 | 6-0-0 | 6-0-0 |
UBC812 | GAGAGAGAGAGAGAGAA | 48.8 | 11-0-0 | 11-0-0 |
UBC818 | CACACACACACACACAG | 53.0 | 5-0-0 | 5-0-0 |
UBC834 | AGAGAGAGAGAGAGAGYT | 54.0 | 7-0-0 | 7-0-0 |
UBC836 | AGAGAGAGAGAGAGAGYA | 51.0 | 7-0-0 | 7-0-0 |
UBC840 | GAGAGAGAGAGAGAGAYT | 54.0 | 10-0-0 | 10-0-0 |
UBC841 | GAGAGAGAGAGAGAGAYC | 46.6 | 5-0-0 | 5-0-0 |
UBC842 | GAGAGAGAGAGAGAGAYG | 48.0 | 5-0-0 | 5-0-0 |
