Figure 1 | Scientific Reports

Figure 1

From: Phosphorylation of endogenous α-synuclein induced by extracellular seeds initiates at the pre-synaptic region and spreads to the cell body

Figure 1

Seed-dependent phosphorylation of α-synuclein in mouse brain. (a) Schematic representation of seed-dependent phosphorylation of α-synuclein after the injection into mouse brain. α-Synuclein PFFs were injected into the CA3c region of mouse hippocampus. Mice were sacrificed after 1 to 14 days, and the brains were fixed and stained with anti-phosphorylated α-synuclein. (b) Appearance of phosphorylated α-synuclein (Phos-αS) in the CA3c region at 3 days (upper), 5 days (middle) or 14 days (lower) after the injection. Images show phosphorylated α-synuclein (green), NeuN (red), a neuronal cell body marker, DAPI (blue) and the merged image in the CA3c region. Regions surrounded by white rectangles in merged images are magnified and shown in the rightmost panel of each lane. (c) Appearance of phosphorylated α-synuclein in the CA3a region at 3 days (upper), 5 days (middle) or 14 days (lower) after the injection. Images show phosphorylated α-synuclein (green), NeuN (red), DAPI (blue) and the merged image in the CA3a region. Regions surrounded by white rectangles in merged images are magnified and shown in the rightmost panel of each lane. Scale bars, 10 μm.

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