Figure 1 | Scientific Reports

Figure 1

From: Serum-free cultures of C2C12 cells show different muscle phenotypes which can be estimated by metabolic profiling

Figure 1The alternative text for this image may have been generated using AI.

The experimental schematic workflow in this study. C2C12 cells were cultured for 7 days in the following 4 different types of mediums. (i) DMEM; only basic medium without any supplementation, (ii) Serum; DMEM supplemented with animal-derived serum. 10% of fetal bovine serum (FBS) and 2% of horse serum (HS) were used for proliferation and differentiation, respectively. (iii) B27; chemically defined serum substitute, 2% and 1% of B27 were supplemented in DMEM for proliferation and differentiation, respectively. (iv) AIM-V, chemically defined medium. Cell morphology and viability were monitored for the entire culture period. Cell growth was further profiled during the proliferation status, CK activity, myotube diameter, muscle contraction were further assessed for myogenic differentiation. Finally, culture samples from day 1 and day 7 representing myoblasts and myotubes were analyzed for the metabolomic study.

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