Figure 6 | Scientific Reports

Figure 6

From: Serum-free cultures of C2C12 cells show different muscle phenotypes which can be estimated by metabolic profiling

Figure 6The alternative text for this image may have been generated using AI.

Principle component analysis (PCA) of intracellular metabolites datasets of C2C12 myoblasts (day 1) and myotubes (day 7) cultured in Serum, B27, and AIM-V. (A) Score plot of PC2 versus PC1. The individual shaded area presents the 95% confidence interval of the group. (B) Biplot. In total, 66% of the data variation is explained by PC1 and PC2. A1, B1, and S1 indicate AIM-V, B27, and Serum culture at day 1, and A7, B7, and S7 indicate AIM-V, B27, and Serum culture at day 7. Significantly increased metabolites in myotubes (day 7) versus myoblasts (day 1) cultured in Serum, B27, and AIM-V mediums. (C) Absolute intracellular concentration (M) of GL3P. (D) Absolute intracellular concentration (M) of UDP-GlcNAc in Serum, B27, and AIM-V culture. (E) The ratio of UDP/UDP-GlcNAc, indicating O-GlcNAcylation. (n) = 4. The student’s t-test was performed to compare day 1 and day 7 in each different culture. *p < 0.05. Data presented as average ± standard deviation (SD).

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