Figure 5
Phage EK99P-1 alleviated the inflammatory response of immune cells under IPEC-J2 infection with ETEC K99. Differentiated IPEC-J2 and pPBMCs were co-cultured using transwell plates. IPEC-J2 were treated with ETEC K99 (1 × 107 cfu/mL) and phage EK99P-1 (1 × 106 pfu/mL) on the apical side for 24 h. (A–C) Expression of cytokine mRNA in pPBMC was measured by real-time quantitative RT-PCR (qRT-PCR; n = 3). (D) pPBMCs were stained with anti-CD172a, -CD3e, -CD4, or -CD8a antibodies to analyze the population of immune cells using flow cytometry. Data are mean percentages of each cell population relative to the total cells ± SD (n = 3). Means were compared using one-way ANOVA, followed by a Friedman test corrected by Tukey’s multiple comparison test. Different letters in each group indicate significant differences at P < 0.05.
