Figure 3 | Scientific Reports

Figure 3

From: Targeting of non-coding RNAs encoded by novel MYC enhancers inhibits the proliferation of human hepatic carcinoma cells in vitro

Figure 3

The eRNA expression by MYC enhancer regions in HepG2 cells. (A) USCS genome browser view of the GRO-seq peak, H3K27ac enrichment, and p300 binding sites along the MYC locus of HepG2 cells. An enlarged display of GRO-seq peak (+ strand and − strand) and H3K27ac enriched reads aligned to the putative enhancer regions (GRCh38/Chr 8: 128,158,808–128,626,390). eRNA primers were designed for six putative enhancer regions (R1, R2, R3, R4, R5, and R6). R1-S, R2-S, etc., stand for the sense strand eRNAs of the R1, R2, etc. regions. The red line above the GRO-seq (+) strand indicates target eRNA. (B) qRT-PCR of eRNA transcription levels in HepG2 cells treated with different concentrations of DRB (10 µM, 25 µM, and 50 µM; 6 h). (C) qRT-PCR of eRNA transcription levels in BET inhibitor-treated HepG2 cells (5 µM, 24 h). The values are the mean ± SD from triplicate well measurements. *p < 0.05 and **p < 0.01.

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