Table 1 Summary of validation experiments for the Sf6p-LAMP assay and the FAW diagnostic protocol.

From: Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay

Experiments

The Sf6p-LAMP assay

The FAW diagnostic protocol

Samples

10 ng genomic DNA of 15 moths (triplicate)

100 genomic DNAs from 50 FAW eggs and 50 S. litura eggs

Positive/false-negative

3 FAW/not-detected

50 FAW eggs/not-detected

Negative/false-positive

14 moths × 3/not-detected

50 S. litura eggs/not-detected

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