Table 1 RT-PCR of the influenza A virus matrix gene-fragment results and virus titers in swabs and organs of H5N8-infected wild Common buzzards (Buteo buteo). *Cycle threshold (Ct) value, cut-off value is 40; ** all titers are given in TCID50 log 10; # this value is likely an outlier; n, negative (< 0.5 for virus titers); np, not present.
RT-PCR values* (virus titers)** | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Samples | Common buzzards | ||||||||||
B1 | B2 | B3 | B4 | B5 | B6 | B7 | B8 | B9 | B10 | B11 | |
Pharyngeal swab | 28 (n) | 30 (n) | 28 (n) | 33 (n) | 23 (n) | 32 (n) | 31 (n) | 36 (n) | 20 (4.8) | 29 (n) | 25 (4) |
Cloacal swab | 32 (n) | 27 (n) | 26 (1.8) | 24 (n) | 27 (n) | 33 (n) | 23 (n) | 26 (n) | 22 (2.8) | 25 (n) | 21 (n) |
Lung | np | 32 | 25 | 25 | 25 | 32 | 31 | 31 | 29 | 20 | 17 |
Air sac | 31 | 25 | np | 24 | 29 | 34 | 30 | 14 | 19 | 22 | 19 |
Liver | 35 | np | 34 | 24 | 25 | 31 | np | 29 | 20 | 28 | 23 |
Spleen | 35 | 24 | 31 | 30 | 31 | 32 | 31 | 29 | 25 | 28 | 21 |
Heart | 24 | 16 | 23 | 31 | 28 | 32 | 31 | 16 | 22 | 18 | 11# |
Kidney | 37 | np | np | 29 | 26 | 31 | 34 | 26 | 26 | 30 | 20 |
Jejunum | 34 | np | 32 | np | 27 | 35 | 34 | 26 | 21 | 35 | 20 |
Colon | np | 34 | 33 | 30 | 31 | 27 | 34 | 30 | 19 | 28 | 23 |
Pancreas | 30 | 34 | np | 26 | 24 | 36 | 35 | 29 | 20 | 32 | 32 |
Brain | 25 | 32 | 21 | 25 | 32 | 31 | 34 | 24 | 24 | 26 | 18 |