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Figure 1

From: Trehangelins ameliorate inflammation-induced skin senescence by suppressing the epidermal YAP-CCN1 axis

Figure 1

Trehangelins (THG) suppress the expression of CCN1 in keratinocytes by inhibiting the translocation of Yes-associated protein (YAP) to the nucleus. Structure of THG A (a). Estimated LogP of THG, Trehalose derivatives and mono/disaccharides was calculated using ChemDraw. Detailed list is shown in Supplementary Information (Table S1) (b). Effects of THG on CCN1 expression. Keratinocytes were pretreated with THG for 24 h and then were stimulated with or without 10 U plasmin, after which they were incubated for another 24 h. The expression of cellular CCN1 from the lysate (c) and secreted CCN1 from the supernatant (d) was determined by Western Blotting and was quantified. Values reported are means ± SD of n = 3 replicates, Dunnett’s test, *p < 0.05, **p < 0.01, ***p < 0.001 (c, d). Immunocytochemistry of YAP and analysis of nuclear YAP-positive cells 0–3 h after Ca or THG treatment (e). Values reported are means of n = 10,000 cells, Bar = 100 μm (e).

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