Figure 4
GENA348 myocardium has an increased mitochondrial density compared to the WT. (A,B) Portions of serial images from the WT SBF-SEM datasets (A) and GENA348 samples highlighting areas (*) of mitochondrial clustering. Scale bar = 5 μm. (C) Analysis of mitochondrial density using a stereological approach sampling SBF-SEM data sets (volume sampled per dataset = 58.4 × 58. 4 × 47.0 μm, n = 3 per group). Mitochondrial volume relative to the cardiomyocyte volume density for WT and GENA348 (mean ± SEM; two-tailed unpaired Student t test; **P < 0.01). (D) Analysis of the mitochondrial subpopulation densities relative to the total mitochondrial shows that IFM are the largest mitochondrial subpopulation in both the WT (left) and GENA348 (right) population. In the WT cardiomyocyte the density of SSM and PNM are similar but in the GENA348 myocardium there are more SSM than PNM. (E) There is no difference in the density of the WT and GENA348 mitochondrial subpopulations relative to the cardiomyocyte density. (F) All mean values of the GENA348 subpopulations are greater than WT, but do not reach significance (2-way ANOVA; Sidaks multiple comparison test) which may be due to the density increase being distributed across all groups.
