Figure 3 | Scientific Reports

Figure 3

From: Experimental inoculation trial to determine the effects of temperature and humidity on White-nose Syndrome in hibernating bats

Figure 3

Examples demonstrate the process of measuring and estimating fungal growth of Pseudogymnoascus destructans (Pd) on media plates in temperature and humidity treatment conditions. (A) Examples of fungal growth on media plates measured at days 7, 14, 21, 28, and 34 from two of the treatment conditions (11 °C, 92% RH and 5 °C, 88% RH). (B) Examples of estimating maximum growth rate and latency variables from fungal growth measurements in panel A. We fit a sigmoidal curve to describe fungal growth (thick solid black line) to estimate the inflection point of the curve (vertical solid line). We calculated the slope (solid red line) at the inflection point of the curve to estimate maximum growth rate, and the days until total growth area reached 2.5 cm2 (dashed red lines) as an estimate of latency.

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